MicroArray Services Overview
OneArray® Gene Expression Services
Phalanx Biotech Group's microarray service lab brings you the very best in Gene Expression Profiling using our proprietary OneArray® Platform, which is continuously validated, tested, and updated. Since we design and manufacture our own OneArray® microarrays in-house, we are able to offer affordable pricing to our customers .
Whole Genome Microarray profiling can be used to analyze the differential expression of genes and often illuminates pathways and biological processes underlying the treatment of interest.
Just send us your RNA or your tissue/cell/serum samples, and we will do the rest! We perform RNA extraction, QC, microarray service, and data analysis.
Highlights
- OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
- 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.
Overview
Species available:
| Species | OneArray Platform | No. of Genome Probes |
|---|---|---|
| Human | HOA v7 | 28,264 |
| Mouse | MOA v2 | 26,423 |
| Rat | ROA v2 | 20,715 |
| Rice | RiOA v1 | 21,179 |
| Yeast | YOA v1 | 6,958 |
Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 3ug |
| RNA QC | NanoDrop | 260/230 > 1.5, 260/280 > 1.8 |
| Agilent Bioanalyzer | RIN > 6 | |
| RNA amplification | Eberwine Method with aa-dUTP incorporation | Internally validated thresholds for aRNA yield measured with NanoDrop |
| RNA labeling | Cy-dye coupling to aa-dUTP | Internally validated thresholds for labeling efficiency measured with NanoDrop |
| Hybridization | Phalanx Method | |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
Data Analysis:
- Data normalization
- Identify differentially expressed genes (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
- Gene Ontology and Pathway enrichment analyses (for each list of differentially expressed genes)
Downloadable Resources
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES
OneArray® microRNA Expression Services
Phalanx Biotech Group's microarray service lab brings you the very best in miRNA Expression Profiling Services using our proprietary OneArray® Platform, which is continuously validated, tested and updated. Since we design and manufacture our own microarrays in-house, we are able to offer affordable pricing to out customers.
Just send us your RNA or your tissue/cell/serum samples, and we will do the rest! We perform RNA extraction, QC, microarray service and data analysis.
Highlights
- OneArray® miRNA expression profiling is available for the following species: Human (HmiOA v7), Mouse & Rat (MRmiOA v7), and Model Plant species (MPmiOA v3)
- Probes contain miRNA-specific sequences and a proprietary spacer designed to enhance the hybridization sensitivity to miRNAs.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, miRNA enrichment, labeling, hybridization, scanning, data extraction, and data analysis.
Overview
Species available:
| Species | Format | Agilent Design ID | No. of Genome Probes |
|---|---|---|---|
| Human | 8 x 60K | 072363 | 26,083 Genes 30,606 lncRNAs |
| Mouse | 8 x 60K | 074809 | 27,122 Genes 4,578 lncRNAs |
| Rat | 8 x 60K | 074036 | 30,584 |
| Arabidopsis | 4 x 44K | 021169 | 43,803 |
| Barley | 4 x 44K | 021623 | 43,803 |
| Bovine | 4 x 44K | 023647 | 43,803 |
| Brassica | 4 x 44K | 022520 | 43,803 |
| C. elegans | 4 x 44K | 020186 | 43,803 |
| Canine | 4 x 44K | 021193 | 43,803 |
| Chicken | 4 x 44K | 026441 | 43,803 |
| Cotton | 4 x 44K | 022523 | 43,803 |
| Drosophila | 4 x 44K | 021791 | 43,803 |
| E. coli | 8 x 15K | 020097 | 15,208 |
| Horse | 4 x 44K | 021322 | 43,803 |
| Magnaporthe | 4 x 44K | 015060 | 15,170 Magnaporthe 6,325 Rice |
| Mosquito | 4 x 44K | 020449 | 43,803 |
| Porcine | 4 x 44K | 026440 | 43,803 |
| Rabbit | 4 x 44K | 020908 | 43,803 |
| Rhesus | 4 x 44K | 026806 | 43,803 |
| Rice | 4 x 44K | 015241 | 43,803 |
| Salmon | 4 x 44K | 020938 | 43,803 |
| Sheep | 8 x 15K | 019921 | 15,208 |
| Tobacco | 4 x 44K | 021113 | 43,803 |
| Tomato | 4 x 44K | 022270 | 43,803 |
| Wheat | 4 x 44K | 022297 | 43,803 |
| Xenopus | 4 x 44K | 023448 | 43,803 |
| Yeast | 8 x 15K | 016322 | 6,256 |
| Zebrafish | 4 x 44K | 026437 | 43,803 |
Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 0.5ug |
| RNA QC | NanoDrop | 260/230 > 0.7, 260/280 > 1.8 |
| Agilent Bioanalyzer | RIN > 7 | |
| RNA amplification and labeling | Agilent Low Input Quick Amp Labeling Kit | Internally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop |
| Hybridization | Agilent Protocol | See below |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
Data Analysis:
- Data normalization
- Identify differentially expressed genes (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
Downloadable Resources
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES
Agilent Microarray Services
Phalanx Biotech Group offers superior gene expression microarray services utilizing industry-leading Agilent microarrays available in 28 different model organisms. Our qualified staff has many years of experience performing Agilent microarray services. Our customer service specialists will help you with experimental design, sample logistics, QC interpretation, hybridization, scanning and data analysis.
Highlights
- OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
- 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
- Microarray service provider for 10+ years.
- Outstanding technical support from microarray experts.
- Professional lab technicians with 70+ years of combined experience.
- Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.
Overview
Species available:
| Species | Format | Agilent Design ID | No. of Genome Probes |
|---|---|---|---|
| Human | 8 x 60K | 072363 | 26,083 Genes 30,606 lncRNAs |
| Mouse | 8 x 60K | 074809 | 27,122 Genes 4,578 lncRNAs |
| Rat | 8 x 60K | 074036 | 30,584 |
| Arabidopsis | 4 x 44K | 021169 | 43,803 |
| Barley | 4 x 44K | 021623 | 43,803 |
| Bovine | 4 x 44K | 023647 | 43,803 |
| Brassica | 4 x 44K | 022520 | 43,803 |
| C. elegans | 4 x 44K | 020186 | 43,803 |
| Canine | 4 x 44K | 021193 | 43,803 |
| Chicken | 4 x 44K | 026441 | 43,803 |
| Cotton | 4 x 44K | 022523 | 43,803 |
| Drosophila | 4 x 44K | 021791 | 43,803 |
| E. coli | 8 x 15K | 020097 | 15,208 |
| Horse | 4 x 44K | 021322 | 43,803 |
| Magnaporthe | 4 x 44K | 015060 | 15,170 Magnaporthe 6,325 Rice |
| Mosquito | 4 x 44K | 020449 | 43,803 |
| Porcine | 4 x 44K | 026440 | 43,803 |
| Rabbit | 4 x 44K | 020908 | 43,803 |
| Rhesus | 4 x 44K | 026806 | 43,803 |
| Rice | 4 x 44K | 015241 | 43,803 |
| Salmon | 4 x 44K | 020938 | 43,803 |
| Sheep | 8 x 15K | 019921 | 15,208 |
| Tobacco | 4 x 44K | 021113 | 43,803 |
| Tomato | 4 x 44K | 022270 | 43,803 |
| Wheat | 4 x 44K | 022297 | 43,803 |
| Xenopus | 4 x 44K | 023448 | 43,803 |
| Yeast | 8 x 15K | 016322 | 6,256 |
| Zebrafish | 4 x 44K | 026437 | 43,803 |
Workflow:
| Step | Method | QC metrics |
|---|---|---|
| RNA isolation (optional) | Trizol/RNeasy/etc. | Need > 0.5ug |
| RNA QC | NanoDrop | 260/230 > 0.7, 260/280 > 1.8 |
| Agilent Bioanalyzer | RIN > 7 | |
| RNA amplification and labeling | Agilent Low Input Quick Amp Labeling Kit | Internally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop |
| Hybridization | Agilent Protocol | See below |
| Data extraction | Agilent Extended Dynamic Range | Internally validated metrics for hybridization efficiency using control microarray probes |
Data Analysis:
- Data normalization
- Identify differentially expressed genes (unlimited statistical comparisons)
- Principal components analysis
- Hierarchical clustering
Downloadable Resources
BROCHURES
SAMPLE REPORTS
SAMPLE SUBMISSION
TECH NOTES
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