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MicroArray Services Overview


PhalanxBio offers a large suite of microarray platforms to fulfill your expression profiling needs. Our professional lab technicians have 17+ years of combined experience, and our rigorous QC processes will ensure that you receive high quality expression data.

OneArray® Gene Expression Services

Phalanx Biotech Group's microarray service lab brings you the very best in Gene Expression Profiling using our proprietary OneArray® Platform, which is continuously validated, tested, and updated. Since we design and manufacture our own OneArray® microarrays in-house, we are able to offer affordable pricing to our customers .

Whole Genome Microarray profiling can be used to analyze the differential expression of genes and often illuminates pathways and biological processes underlying the treatment of interest.

Just send us your RNA or your tissue/cell/serum samples, and we will do the rest! We perform RNA extraction, QC, microarray service, and data analysis.

Highlights

  • OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
  • 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.

Overview

Species available:
SpeciesOneArray PlatformNo. of Genome Probes
HumanHOA v728,264
MouseMOA v226,423
RatROA v220,715
RiceRiOA v121,179
YeastYOA v16,958
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 3ug
RNA QCNanoDrop260/230 > 1.5, 260/280 > 1.8
 Agilent BioanalyzerRIN > 6
RNA amplificationEberwine Method with aa-dUTP incorporationInternally validated thresholds for aRNA yield measured with NanoDrop
RNA labelingCy-dye coupling to aa-dUTPInternally validated thresholds for labeling efficiency measured with NanoDrop
HybridizationPhalanx Method 
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering
  • Gene Ontology and Pathway enrichment analyses (for each list of differentially expressed genes)

Downloadable Resources

OneArray® microRNA Expression Services

Phalanx Biotech Group's microarray service lab brings you the very best in miRNA Expression Profiling Services using our proprietary OneArray® Platform, which is continuously validated, tested and updated. Since we design and manufacture our own microarrays in-house, we are able to offer affordable pricing to out customers.
Just send us your RNA or your tissue/cell/serum samples, and we will do the rest! We perform RNA extraction, QC, microarray service and data analysis.

Highlights

  • OneArray® miRNA expression profiling is available for the following species: Human (HmiOA v7), Mouse & Rat (MRmiOA v7), and Model Plant species (MPmiOA v3)
  • Probes contain miRNA-specific sequences and a proprietary spacer designed to enhance the hybridization sensitivity to miRNAs.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, miRNA enrichment, labeling, hybridization, scanning, data extraction, and data analysis.

Overview

Species available:
SpeciesFormatAgilent Design IDNo. of Genome Probes
Human8 x 60K07236326,083 Genes 30,606 lncRNAs
Mouse8 x 60K07480927,122 Genes 4,578 lncRNAs
Rat8 x 60K07403630,584
Arabidopsis4 x 44K02116943,803
Barley4 x 44K02162343,803
Bovine4 x 44K02364743,803
Brassica4 x 44K02252043,803
C. elegans4 x 44K02018643,803
Canine4 x 44K02119343,803
Chicken4 x 44K02644143,803
Cotton4 x 44K02252343,803
Drosophila4 x 44K02179143,803
E. coli8 x 15K02009715,208
Horse4 x 44K02132243,803
Magnaporthe4 x 44K01506015,170 Magnaporthe 6,325 Rice
Mosquito4 x 44K02044943,803
Porcine4 x 44K02644043,803
Rabbit4 x 44K02090843,803
Rhesus4 x 44K02680643,803
Rice4 x 44K01524143,803
Salmon4 x 44K02093843,803
Sheep8 x 15K01992115,208
Tobacco4 x 44K02111343,803
Tomato4 x 44K02227043,803
Wheat4 x 44K02229743,803
Xenopus4 x 44K02344843,803
Yeast8 x 15K0163226,256
Zebrafish4 x 44K02643743,803
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 0.5ug
RNA QCNanoDrop260/230 > 0.7, 260/280 > 1.8
 Agilent BioanalyzerRIN > 7
RNA amplification and labelingAgilent Low Input Quick Amp Labeling KitInternally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop
HybridizationAgilent ProtocolSee below
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering

Downloadable Resources

Agilent Microarray Services

Phalanx Biotech Group offers superior gene expression microarray services utilizing industry-leading Agilent microarrays available in 28 different model organisms. Our qualified staff has many years of experience performing Agilent microarray services. Our customer service specialists will help you with experimental design, sample logistics, QC interpretation, hybridization, scanning and data analysis.

Highlights

  • OneArray® gene expression profiling is available for the following species: Human (HOA), Mouse (MOA), Rat (ROA), Rice (RiOA), and Yeast (YOA).
  • 60-mer probes were engineered using specific lengths to match melting temperatures for superior hybridization performance.
  • Microarray service provider for 10+ years.
  • Outstanding technical support from microarray experts.
  • Professional lab technicians with 70+ years of combined experience.
  • Our complete microarray service workflow includes RNA QC, amplification, labeling, hybridization, scanning, data extraction, and data analysis.

Overview

Species available:
SpeciesFormatAgilent Design IDNo. of Genome Probes
Human8 x 60K07236326,083 Genes 30,606 lncRNAs
Mouse8 x 60K07480927,122 Genes 4,578 lncRNAs
Rat8 x 60K07403630,584
Arabidopsis4 x 44K02116943,803
Barley4 x 44K02162343,803
Bovine4 x 44K02364743,803
Brassica4 x 44K02252043,803
C. elegans4 x 44K02018643,803
Canine4 x 44K02119343,803
Chicken4 x 44K02644143,803
Cotton4 x 44K02252343,803
Drosophila4 x 44K02179143,803
E. coli8 x 15K02009715,208
Horse4 x 44K02132243,803
Magnaporthe4 x 44K01506015,170 Magnaporthe 6,325 Rice
Mosquito4 x 44K02044943,803
Porcine4 x 44K02644043,803
Rabbit4 x 44K02090843,803
Rhesus4 x 44K02680643,803
Rice4 x 44K01524143,803
Salmon4 x 44K02093843,803
Sheep8 x 15K01992115,208
Tobacco4 x 44K02111343,803
Tomato4 x 44K02227043,803
Wheat4 x 44K02229743,803
Xenopus4 x 44K02344843,803
Yeast8 x 15K0163226,256
Zebrafish4 x 44K02643743,803
Workflow:
StepMethodQC metrics
RNA isolation (optional)Trizol/RNeasy/etc.Need > 0.5ug
RNA QCNanoDrop260/230 > 0.7, 260/280 > 1.8
 Agilent BioanalyzerRIN > 7
RNA amplification and labelingAgilent Low Input Quick Amp Labeling KitInternally validated thresholds for aRNA yield and labeling efficiency measured with NanoDrop
HybridizationAgilent ProtocolSee below
Data extractionAgilent Extended Dynamic RangeInternally validated metrics for hybridization efficiency using control microarray probes
Data Analysis:
  • Data normalization
  • Identify differentially expressed genes (unlimited statistical comparisons)
  • Principal components analysis
  • Hierarchical clustering

Downloadable Resources